Not established

Not established

Course competences
Code	Description
E01	Express themselves correctly in basic biological, physical, chemical, mathematical and computer terms.
E13	Correct handling of different computer tools
E18	To know the principles of the manipulation of nucleic acids, as well as the techniques that allow the study of the gene function and the development of transgenic organisms with applications in biomedicine, industry, environment, agriculture, etc.
G01	To possess and understand the knowledge in the area of Biochemistry and Molecular Biology at a level that, based on advanced textbooks, also includes cutting-edge aspects of relevance in the discipline
G02	To know how to apply the knowledge of Biochemistry and Molecular Biology to professional practice and to possess the necessary intellectual skills and abilities for this practice, including the capacity for: information management, analysis and synthesis, problem solving, organization and planning and generation of new ideas.
G03	Be able to collect and interpret relevant data, information and results, draw conclusions and issue reasoned reports on relevant social, scientific or ethical issues in connection with advances in Biochemistry and Molecular Biology.
G04	To know how to transmit information, ideas, problems and solutions in the field of Biochemistry and Molecular Biology to a specialized and non-specialized public.
G05	Develop those strategies and learning skills necessary to undertake further studies in the area of Biochemistry and Molecular Biology and other related areas with a high degree of autonomy.
G06	Acquire skills in the handling of computer programs including access to bibliographic, structural or any other type of databases useful in Biochemistry and Molecular Biology.
T01	Proficiency in a second foreign language, preferably English, at level B1 of the Common European Framework of Reference for Languages
T02	User-level knowledge of Information and Communication Technologies (ICT).
T04	Ethical commitment and professional deontology
T08	Ability to work as a team and, where appropriate, exercise leadership functions, encouraging entrepreneurship

Course learning outcomes
Description
To be able to express oneself correctly with the appropriate terms about the different genetic and molecular processes that occur in the cell.
Solve and design experiments in the field of Molecular Biology.
Know how to apply molecular techniques for the identification and genotyping of microorganisms of industrial interest.
To know the basic fundamentals of the most frequent instrumentation in Molecular Biology laboratories.
Acquire the basic concepts necessary for the use of recombinant DNA technology.
To understand the potential applications of molecular biotechnology in agriculture, food, medicine, environment and industry and the main current trends and future challenges.
Acquire the necessary scientific criteria to develop professional ethics in the application of genetic engineering and biotechnology.
To understand the molecular mechanisms responsible for gene expression and its regulation in prokaryotes and eukaryotes.
Become familiar with the scientific literature and with the search for and communication of scientific information.
To know the techniques used to obtain genetically modified microorganisms, plants and animals.
Additional outcomes
Description

• Unit 1: Monitoring of starter culture in yoghurt fermentation
  • Unit 1.1: preparation of media and reagents
  • Unit 1.2: Use of RAPD-PCR technique
• Unit 2: Generation of mutant versions of D-lactate dehydrogenase
  • Unit 2.1: Cloning strategy
  • Unit 2.2: Primer design
  • Unit 2.3: Cloning of wild-type and mutant versions of Lactate dehydrogenase
• Unit 3: Purification and functional characterization of the wild and mutant versions of D-lactate DH obtained.
  • Unit 3.1: Expression and purification of the wild and mutant versions of D-lactate DH obtained as fusion proteins
  • Unit 3.2: Determination of LDH activity in the obtained versions
  • Unit 3.3: Detection of recombinant proteins by Western-blot

Training Activity	Methodology	Related Competences (only degrees before RD 822/2021)	ECTS	Hours	As	Com	Description
Class Attendance (practical) [ON-SITE]	Practical or hands-on activities	T01	2.04	51	Y	Y	Laboratory practices. Assistance is compulsory and non-reschedulable
Workshops or seminars [ON-SITE]	Workshops and Seminars	E01 E13 E18 G04	0.24	6	Y	N	Completion of task related with the experimental design. Non-reschedulable.
Practicum and practical activities report writing or preparation [OFF-SITE]	Self-study	E01 E13 G03	2	50	Y	N	Practical activities report. Non-reschedulable
Other off-site activity [OFF-SITE]	Reading and Analysis of Reviews and Articles	E01 E13 G03	0.4	10	N	N	Report preparation
Study and Exam Preparation [OFF-SITE]	Self-study	E01 E13 G03	1.2	30	N	N	Preparation of final test
Final test [ON-SITE]	Assessment tests	E01 G04	0.12	3	Y	Y	Final test of the subject. Reschedulable
Total:			6	150
Total credits of in-class work: 2.4			Total class time hours: 60
Total credits of out of class work: 3.6			Total hours of out of class work: 90

As: Assessable training activity
Com: Training activity of compulsory overcoming (It will be essential to overcome both continuous and non-continuous assessment).

Evaluation System	Continuous assessment	Non-continuous evaluation *	Description
Final test	74.00%	80.00%	Final exam. Reschedulable
Other methods of assessment	6.00%	0.00%	Student will submit answer to several cuestionnaires. Non-reschedulable
Practicum and practical activities reports assessment	20.00%	20.00%	Students will prepare a report of the practices carried out in the laboratory, in which the results obtained will be described and discussed. The report also will include an abstract of the work done. Non-reschedulable
Total:	100.00%	100.00%

Not related to the syllabus/contents
Hours	hours

Author(s)	Title	Book/Journal	Citv	Publishing house	ISBN	Year	Description	Link	Catálogo biblioteca
	Algoritmo para la generación de alineamientos múltiples (ClustalW)							http://www.ebi.ac.uk/clustalw/index.html
	Programa de visualización de estructuras tridimensionales (RasMol)							http://rasmol.org/
	Protein Data Bank							http://www.rcsb.org/pdb/home/home.do
	Uniprot							http://www.uniprot.org/
A. Herraez	Biología Molecular e Ingeniería Genética				978-848086-647-7	2012
Brock, Thomas D.	Brock, biología de los microorganismos			Prentice Hall	84-89660-36-0	2001
Green and Sambrook	Molecular Cloning. A laboratory manual. 4th edition				978-1936113422	2012
Yousef, Ahmed E.	Microbiología de los alimentos : manual de laboratorio			Acribia	10-84-200-1066-9	2006