Guías Docentes Electrónicas
1. General information
Course:
INSTRUMENTAL SEPARATION METHODS
Code:
57321
Type:
CORE COURSE
ECTS credits:
6
Degree:
409 - CHEMISTRY
Academic year:
2022-23
Center:
1 - FACULTY OF SCIENCE AND CHEMICAL TECHNOLOGY
Group(s):
20  23 
Year:
3
Duration:
C2
Main language:
Spanish
Second language:
Use of additional languages:
English Friendly:
Y
Web site:
Bilingual:
N
Lecturer: GREGORIO CASTAÑEDA PEÑALVO - Group(s): 20  23 
Building/Office
Department
Phone number
Email
Office hours
San Alberto Magno/planta baja
Q. ANALÍTICA Y TGIA. ALIMENTOS
3445
gregorio.castaneda@uclm.es
Monday, Tuesday and Thursday from 11:30 to 13:30. Request an appointment by email.

Lecturer: MARIA JESUS VILLASEÑOR LLERENA - Group(s): 20  23 
Building/Office
Department
Phone number
Email
Office hours
Politécnico/A24
Q. ANALÍTICA Y TGIA. ALIMENTOS
926052673
mjesus.villasenor@uclm.es
M,T,T de 13:00 to 15:00 h

2. Pre-Requisites

Not established

It is recommended to have studied the subjects: Fundamentals of analytical chemistry, instrumental analysis I and instrumental analysis II

3. Justification in the curriculum, relation to other subjects and to the profession

The subject of instrumental methods of separation is included within the subject of instrumental analysis, including in the Chemistry Fundamentals module. Its justification in the curriculum is obvious due to analytical separation techniques are one of the most powerful tools available to carry out the qualitative and quantitative determination of the analytes of interest in  different samples (pharmaceutical, biological, environmental, etc.).  In this subject we study the theoretical principles of the different instrumental separation techniques, with special emphasis on chromatographic techniques.  It will also address the study of the different instrumentation components. An important aspect to consider will be the different detectors that can be used, the hybridization with the mass spectrometry and the expected response according to the selectivity or universality of the detector.

Finally, this subject is related to the other two that configure the matter of instrumental analysis (instrumental analysis I and instrumental analysis II). This ubject contributes to carry out the functions of the chemical graduate in the Laboratory of Quality control and in the research and development laboratories.


4. Degree competences achieved in this course
Course competences
Code Description
CB02 Apply their knowledge to their job or vocation in a professional manner and show that they have the competences to construct and justify arguments and solve problems within their subject area.
CB03 Be able to gather and process relevant information (usually within their subject area) to give opinions, including reflections on relevant social, scientific or ethical issues.
CB04 Transmit information, ideas, problems and solutions for both specialist and non-specialist audiences.
CB05 Have developed the necessary learning abilities to carry on studying autonomously
E05 Know the chemical elements and their compounds, their forms of obtaining, structure, properties and reactivity, as well as the main techniques for their analysis
E06 Know the structural properties of chemical compounds, including stereochemistry, as well as the main structural research techniques
E14 Know and know how to apply the metrology of chemical processes, including quality management
E15 Know how to handle the standard chemical instrumentation and be able to elaborate and manage standardized procedures of work in the laboratory and chemical industry
E16 Plan, design and develop projects and experiments
E17 Develop the ability to relate to each other the different specialties of Chemistry, as well as this one with other disciplines (interdisciplinary character)
G02 Be able to gather and interpret data, information and relevant results, obtain conclusions and issue reasoned reports on scientific, technological or other problems that require the use of chemical tools
G03 Know how to apply the theoretical-practical knowledge acquired in the different professional contexts of Chemistry
G04 Know how to communicate, orally and in writing, the knowledge, procedures and results of chemistry, both specialized and non-specialized
G05 Acquire and adapt new knowledge and techniques of any scientific-technical discipline with incidence in the chemical field
T04 Ethical commitment and professional ethics
T05 Organization and planning capacity
T06 Ability to approach decision making
T09 Motivation for quality, job security and awareness of environmental issues, with knowledge of internationally recognized systems for the correct management of these aspects
T10 Ability to use specific software for chemistry at user level
T11 Ability to obtain bibliographic information, including Internet resources
5. Objectives or Learning Outcomes
Course learning outcomes
Description
Know the essential components of the current analytical instrumentation and its function within the configuration of the instrument
Know the basics of the main instrumental analytical techniques and their most relevant applications currently in laboratories
Train the student to tackle an analytical problem, search and select the most relevant bibliography, synthesize it by extracting its most important parts, and expose and explain it in public
Train the student to be sensitive to the ethical exercise of the profession, becoming aware of the social responsibility of their reports and their impact on decision making
Acquire skills for practical laboratory work, being able to develop experimentally analytical processes that include the planning of sampling, its treatment and analysis using different instrumental methods, emphasizing its use to solve real analytical problems
Acquire skills for planning, writing and validating work protocols in the laboratory
Acquire critical judgment in the selection of the most appropriate analytical methodology according to the usual standards
Understand the advantages, disadvantages, limitations and applications of the main instrumental methods of analysis
Additional outcomes
Description
Train the student to select the most appropriate separation technique to perform the analys, depending on the type of sample and analyte to be determined.
6. Units / Contents
  • Unit 1: Introduction to separation techniques
  • Unit 2: Gas chromatography
  • Unit 3: Thin layer Chromatography
  • Unit 4: Liquid chromatography
  • Unit 5: Liquid liquid partition chormatography
  • Unit 6: Adsorption chromatography
  • Unit 7: Size exclusion cromatography
  • Unit 8: Ion exchange chromatography
  • Unit 9: Supercritical fluid Chromatography
  • Unit 10: Capillary electrophoresis
7. Activities, Units/Modules and Methodology
Training Activity Methodology Related Competences ECTS Hours As Com Description
Class Attendance (theory) [ON-SITE] Lectures E05 E06 E14 E17 G02 G03 G04 G05 T11 1.08 27 N N The fundamental aspects of the subject will be exposed, interacting with the students through questions that improve the monitoring of the contents covered.
Class Attendance (practical) [ON-SITE] Practical or hands-on activities E05 E06 E14 E15 E16 E17 G02 G03 G04 G05 T10 0.64 16 Y Y There will be practices related to the theory topics covered in class.
Workshops or seminars [ON-SITE] Problem solving and exercises E06 E14 E16 E17 G02 G03 G04 G05 T10 T11 0.4 10 Y N Throughout the semester exercises and problems will be done at the end of each topic or block of theoretical topics.
Group tutoring sessions [ON-SITE] Group tutoring sessions E05 E14 E17 G03 G04 0.08 2 N N Discussion and resolution of concepts and questions
Study and Exam Preparation [OFF-SITE] Self-study E05 E06 E14 E16 E17 G02 G03 G04 G05 T10 T11 0.96 24 N N Obtain documentation, preparation, learning and testing the resolution of practical cases.
Mid-term test [ON-SITE] Assessment tests E16 G02 G03 G04 0.08 2 Y N They will consist of solving theoretical and practical questions and graphical-numerical problems.
Final test [ON-SITE] Assessment tests E16 G02 G03 G04 0.12 3 Y Y Final evaluation written test. They will consist of solving theoretical and practical questions and graphical-numerical problems.
Writing of reports or projects [OFF-SITE] Self-study 1.64 41 Y Y Problem solving and practical cases
Practicum and practical activities report writing or preparation [OFF-SITE] Self-study 1 25 Y Y
Total: 6 150
Total credits of in-class work: 2.4 Total class time hours: 60
Total credits of out of class work: 3.6 Total hours of out of class work: 90

As: Assessable training activity
Com: Training activity of compulsory overcoming (It will be essential to overcome both continuous and non-continuous assessment).

8. Evaluation criteria and Grading System
Evaluation System Continuous assessment Non-continuous evaluation * Description
Mid-term tests 35.00% 0.00% A short trial will be conducted during the semester to assess learning follow-up
Laboratory sessions 15.00% 15.00% Laboratory Work and report
Assessment of problem solving and/or case studies 15.00% 0.00% The student's circulatory problems resolution and participation in the class will be positively valued
Final test 35.00% 85.00% A final exam of the subject will be carried out. Those students who have passed the test of progress will be evaluated from the rest of the subject.
Total: 100.00% 100.00%  
According to art. 6 of the UCLM Student Evaluation Regulations, it must be provided to students who cannot regularly attend face-to-face training activities the passing of the subject, having the right (art. 13.2) to be globally graded, in 2 annual calls per subject , an ordinary and an extraordinary one (evaluating 100% of the competences).

Evaluation criteria for the final exam:
  • Continuous assessment:
    Laboratory assistance is obligatory. In order to be able to apply the valuation percentages indicated in the evaluation system, it is necessary to obtain at least a 4 in the final test and in the laboratory practices.
    If the above requirements are not met, the qualification will be fail.
  • Non-continuous evaluation:
    A final theoric exam (85%) and laboratory (15%)will be done. It Will be necessary to obtain a rating greater than 4 in the written exam and to the laboratory practices.

Specifications for the resit/retake exam:
A final written exam will be held (85%) and the qualification obtained from the laboratory practices (15%), either in the performance of the same or in the test authorized to pass them, will be considered. In order to apply the percentages indicated, it is necessary to obtain a score higher than 4 out of 10 in both the written exam and the laboratory practice exam.
Specifications for the second resit / retake exam:
The same of the retake exam.
9. Assignments, course calendar and important dates
Not related to the syllabus/contents
Hours hours
Class Attendance (practical) [PRESENCIAL][Practical or hands-on activities] 16
Workshops or seminars [PRESENCIAL][Problem solving and exercises] 10
Group tutoring sessions [PRESENCIAL][Group tutoring sessions] 2
Study and Exam Preparation [AUTÓNOMA][Self-study] 24
Mid-term test [PRESENCIAL][Assessment tests] 2
Final test [PRESENCIAL][Assessment tests] 3
Writing of reports or projects [AUTÓNOMA][Self-study] 41
Practicum and practical activities report writing or preparation [AUTÓNOMA][Self-study] 25

Unit 1 (de 10): Introduction to separation techniques
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 3

Unit 2 (de 10): Gas chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 5

Unit 3 (de 10): Thin layer Chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 3

Unit 4 (de 10): Liquid chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 2

Unit 5 (de 10): Liquid liquid partition chormatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 2

Unit 6 (de 10): Adsorption chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 2

Unit 7 (de 10): Size exclusion cromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 2

Unit 8 (de 10): Ion exchange chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 1

Unit 9 (de 10): Supercritical fluid Chromatography
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 2

Unit 10 (de 10): Capillary electrophoresis
Activities Hours
Class Attendance (theory) [PRESENCIAL][Lectures] 5

Global activity
Activities hours
10. Bibliography and Sources
Author(s) Title Book/Journal Citv Publishing house ISBN Year Description Link Catálogo biblioteca
 
 
 
 
A. Braithwaite Chromatographic methods New York Chapman and Hall 0-412-25890-0 1990  
Cela R; Lorenzo R.A; Casais M.C. tecnicas de separación en Química Analítica Síntesis 2002  
Dabrio, M.V. Cromatografía y electroforesis en columna Springer-Verlag Ibérica 2000  
Fernández Gutierrez A; Segura Carretero A. Electroforesis capilar: Aproximación sugún la técnica de detección Universidad de Granada 2005  
Hernández L.; González, C. Introducción al análisis instrumental Ed. Ariel 2002  
Hobart H. Willard. INSTRUMENTAL methods of analysis Libro Wadsworth Belmont, California : 0-534-98144-5 1988  
Patrick Camilleri Capillary electrophoresis : theory and practice / edited by Patrick Camilleri Boca Raton CRC Press 0-8493-9127-X 1997  
Rubinson K. A.; Rubinson J.F. Análisis Instrumental Prentice Hall 2000  
Skoog, D. A; Holler, F. J.; Nieman, T. A. Principios de Análisis Instrumental McGraw-Hill 2000  
Skoog, D.; West, D.; Holler, F.; Crouch, S. Fundamentos de Química Analítica Thomson 2005  
Valcárcel Cases M; Gómez Hens A. Técnicas Analíticas de Separación Reverté 1990  



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