1. General information
Course:
METHODOLOGY AND INSTRUMENTATION IN BIOCHEMISTRY
Code:
13309
Type:
CORE COURSE
ECTS credits:
6
Degree:
341 - UNDERGRADUATE DEGREE PROGRAMME IN BIOCHEMISTRY
Academic year:
2021-22
Center:
501 - FACULTY OF ENVIRONMENTAL SCIENCES AND BIOCHEMISTRY
Group(s):
40
Year:
2
Duration:
First semester
Main language:
Spanish
Second language:
Use of additional languages:
English Friendly:
Y
Web site:
Bilingual:
N
Lecturer:
MARIA JIMENEZ MORENO
- Group(s):
40
Building/Office
Department
Phone number
Email
Office hours
Sabatini/0.8
Q. ANALÍTICA Y TGIA. ALIMENTOS
926051710
maria.jimenez@uclm.es
Tuesday, Wednesday and Thursday from 12 to 14 h. Arrange an appointment by e-mail
Lecturer:
NURIA RODRIGUEZ FARIÑAS
- Group(s):
40
Building/Office
Department
Phone number
Email
Office hours
Sabatini/0.9
Q. ANALÍTICA Y TGIA. ALIMENTOS
5459
nuria.rodriguez@uclm.es
Monday, Tuesday and Wednesday from 12 to 14 h (to arrange an appointment by e-mail).
Lecturer:
ROSA DEL CARMEN RODRIGUEZ MARTIN-DOIMEADIOS
- Group(s):
40
Building/Office
Department
Phone number
Email
Office hours
Sabatini/0.16
Q. ANALÍTICA Y TGIA. ALIMENTOS
5420
rosacarmen.rodriguez@uclm.es
Monday, Tuesday and Wednesday from 13 to 14 h and from 15 to 16 h. Arrange an appointment by e-mail
2. Pre-Requisites
Not established
3. Justification in the curriculum, relation to other subjects and to the profession
It is a compulsory subject that belongs to the area of "Instrumental Methodologies" and, within this, to the module "Biochemical Methods and Molecular Biology of Systems". This course will cover the different stages of the analytical process and provide an overview of the methods of analysis and the most important instrumental analysis techniques, with special emphasis on their biochemical application.
Two types of relationships can be established with the other subjects of the Degree. On the one hand, the group of subjects corresponding to the basic sciences, such as Chemistry, Physics and Mathematics, which will be useful in establishing the fundamentals and applications of the techniques studied. On the other hand, those subjects for which the knowledge acquired in this subject will be an especially useful tool. It is directly related to the subjects "Integrated Laboratory I", "Structural Determination", "Clinical Biochemistry", "Molecular Biology of Systems and Bioinformatics" and "Bioethics, Biosafety and Quality Control", as well as those Subjects of Biomedical Guidance and Clinic, especially with "Clinical Bioanalytics".
Regarding the relationship with the profession, basic tools that have their application in very different fields of professional activity will be we studied, although their most direct application is in the professional profile corresponding to the health field and clinical analysis laboratories.
4. Degree competences achieved in this course
| Course competences | |
|---|---|
| Code | Description |
| E01 | Express themselves correctly in basic biological, physical, chemical, mathematical and computer terms. |
| E02 | Work properly and quality driven in a chemical, biological and biochemical laboratory, including safety, handling and disposal of waste and keeping a record of activities. |
| E04 | To know the principles and applications of the methods and instrumentation used in bioanalytical determinations. |
| E12 | Have the numerical and computational skills to apply mathematical procedures for data analysis. |
| E13 | Correct handling of different computer tools |
| T01 | Proficiency in a second foreign language, preferably English, at level B1 of the Common European Framework of Reference for Languages |
| T03 | A correct oral and written communication |
| T05 | Organizational and planning skills |
| T08 | Ability to work as a team and, where appropriate, exercise leadership functions, encouraging entrepreneurship |
5. Objectives or Learning Outcomes
| Course learning outcomes | |
|---|---|
| Description | |
| Understand the physico-chemical principles on which the main instrumental analysis techniques are based. | |
| To be able to estimate the reliability of the analytical results, having a clear idea of the statistical concepts applied for their evaluation, and to understand the validation strategies of the analytical methodologies. | |
| To be able to select the most suitable technique for the analytical study of substances of biochemical interest. | |
| Be able to take and treat samples of a biochemical nature for monitoring and analysis. | |
| Be able to analyse, interpret and draw conclusions from experimental data. | |
| Understand correctly the functioning of the basic instrumentation used in biochemical research | |
| Acquire the necessary skills to use relevant techniques in biochemistry. | |
| Additional outcomes | |
| Not established. |
6. Units / Contents
-
Unit 1: Introduction.
-
Unit 1.1: Introduction to biochemical analysis.
-
Unit 1.2: Analytical properties. Validation and quality.
-
Unit 1.3: Treatment and preparation of samples.
-
-
Unit 2: Spectroscopic techniques.
-
Unit 2.1: Introduction to spectroscopic techniques.
-
Unit 2.2: UV-visible molecular absorption spectrophotometry.
-
Unit 2.3: Molecular fluorescence.
-
Unit 2.4: Atomic spectroscopic techniques.
-
-
Unit 3: Electrochemical techniques.
-
Unit 3.1: Introduction to electrochemical techniques.
-
Unit 3.2: Potentiometry.
-
-
Unit 4: Separation techniques.
-
Unit 4.1: Introduction to separation techniques and chromatography.
-
Unit 4.2: Gas chromatography.
-
Unit 4.3: Liquid chromatography (I): Instrumentation.
-
Unit 4.4: Liquid chromatography (II): Modalities.
-
Unit 4.5: Electrophoresis.
-
-
Unit 5: Mass spectrometry.
-
Unit 5.1: Introduction to mass spectrometry.
-
Unit 5.2: Instrumentation and applications.
-
-
Unit 6: Laboratory Sessions.
-
Unit 6.1: Spectrophotometric determination of phosphates in biological matrices.
-
Unit 6.2: Potentiometric determination in biological fluids.
-
7. Activities, Units/Modules and Methodology
| Training Activity | Methodology | Related Competences (only degrees before RD 822/2021) | ECTS | Hours | As | Com | Description | |
| Class Attendance (practical) [ON-SITE] | Practical or hands-on activities | E01 E02 E04 E12 E13 T03 T08 | 0.32 | 8 | Y | Y | Attendance to the laboratory sessions will be compulsory. The student`s ability and aptitude as well as their ability to work in a group will be evaluated in these practical sessions. This activity is compulsory and not recoverable. The evaluation of this activity could be recover in the different calls. | |
| Practicum and practical activities report writing or preparation [OFF-SITE] | Group Work | E01 E12 E13 T03 T05 T08 | 0.22 | 5.5 | Y | Y | A report about the lab sessiones will be delivered in due time and form. | |
| Other on-site activities [ON-SITE] | Assessment tests | E01 E04 E12 E13 | 0.03 | 0.75 | Y | Y | A written evaluation test about laboratory sessions will be performed. | |
| Study and Exam Preparation [OFF-SITE] | 0.03 | 0.75 | N | N | ||||
| Class Attendance (theory) [ON-SITE] | Lectures | E01 E04 E12 T01 | 1.4 | 35 | N | N | Teaching classes will be developed in an interactive way with the students including open discussion. The presentations to follow the classes will be available at the Moodle virtual platform for download. | |
| Study and Exam Preparation [OFF-SITE] | 2.4 | 60 | N | N | ||||
| Problem solving and/or case studies [ON-SITE] | Problem solving and exercises | E01 E04 E12 E13 T01 T05 | 0.36 | 9 | N | N | These sessions pretend to improve the comprehension of the studied topics through the resolution of numerical exercises and cases studies. | |
| Study and Exam Preparation [OFF-SITE] | 0.7 | 17.5 | N | N | ||||
| Final test [ON-SITE] | Assessment tests | E01 E12 E13 | 0.12 | 3 | Y | Y | A final written test about resolution of problems or exercises will be performed. This final test will evaluate problem solving ability. | |
| Final test [ON-SITE] | Assessment tests | E01 E04 T01 T03 | 0.16 | 4 | Y | Y | A final written test will be performed. This final test will evaluate theoretical contents of the subject. | |
| Other off-site activity [OFF-SITE] | Problem solving and exercises | E01 E04 E12 | 0.25 | 6.25 | Y | N | During the course, proposed problems will be solved and delivered within the indicated period of time. This activity could not be re-taken. | |
| Other on-site activities [ON-SITE] | Practical or hands-on activities | E01 E04 | 0.01 | 0.25 | Y | Y | An initial evaluation test will be performed before the lab sessions. This activity will be compulsory and unrecoverable. | |
| Total: | 6 | 150 | ||||||
| Total credits of in-class work: 2.4 | Total class time hours: 60 | |||||||
| Total credits of out of class work: 3.6 | Total hours of out of class work: 90 | |||||||
As: Assessable training activity Com: Training activity of compulsory overcoming (It will be essential to overcome both continuous and non-continuous assessment).
8. Evaluation criteria and Grading System
| Evaluation System | Continuous assessment | Non-continuous evaluation * | Description |
| Final test | 20.00% | 25.00% | This final exam will consist in a written test about the problems solving ability. To average this mark with those obtained in the rest of activities, it will be necessary to obtain in the final test a minimum score of 4 points out of 10. To pass the subject, a minimum mark of 4 points out of 10 will be required. |
| Final test | 50.00% | 55.00% | This final exam will consist in a written test about the theoretical contents of the subject. To average this mark with those obtained in the rest of activities, it will be necessary to obtain in the final test a minimum score of 4 points out of 10. To pass the subject, a minimum mark of 4 points out of 10 will be required. |
| Practicum and practical activities reports assessment | 7.50% | 7.50% | The delivery of the lab work report will be compulsory to pass the laboratory sessions. This delivery will be done within the expected period (normally the following week after the completion of the laboratory sessions). To pass this activity a minimum grade of 4.0 points out of 10 will be required. |
| Laboratory sessions | 2.50% | 2.50% | An initial test will be performed before the lab sessions in order to assess the comprehension about the lab guidelines. This activity will be compulsory and unrecoverable. |
| Test | 10.00% | 10.00% | A written evaluation test about the laboratory sessions will be performed. This activity will be compulsory and could be re-taken. To average with the rest of the laboratory marks and pass the subject, a minimum grade of 4 out to 10 will be required. |
| Assessment of problem solving and/or case studies | 10.00% | 0.00% | During the course, problems and/or cases studies will be solved and delivered within the indicated period of time. This activity could not be re-taken. |
| Total: | 100.00% | 100.00% |
According to art. 4 of the UCLM Student Evaluation Regulations, it must be provided to students who cannot regularly attend face-to-face training activities the passing of the subject, having the right (art. 12.2) to be globally graded, in 2 annual calls per subject , an ordinary and an extraordinary one (evaluating 100% of the competences).
Evaluation criteria for the final exam:
-
Continuous assessment:In order to pass the subject, it will be compulsory to have attended all the laboratory sessions and a minimum grade of 4.0 out of 10 in the evaluation test about the lab sessions.
For the calculation of the final grade, the marks obtained in the different activities developed during the course will be considered, but only if a minimum grade of 4.0 out of 10 is obtained in the final test. The global mark of the subject will be calculated taking into account the percentages of the previous table. To pass the subject it will be compulsory to obtain a minimum grade of 5 points out of 10 in the global mark. -
Non-continuous evaluation:In order to pass the subject, it will be compulsory to have attended all the laboratory sessions and a minimum grade of 4.0 out of 10 in the evaluation test about the lab sessions.
For the calculation of the final grade, the marks obtained in the different activities developed during the course will be considered, but only if a minimum grade of 4.0 out of 10 is obtained in the final test. The global mark of the subject will be calculated taking into account the percentages of the previous table. To pass the subject it will be compulsory to obtain a minimum grade of 5 points out of 10 in the global mark.
Specifications for the resit/retake exam:
For the retake exam call only the final test and the laboratory sessions evaluation test can be re-taken, applying for the other sections the marks obtained during the development of the course.
Specifications for the second resit / retake exam:
For the special retake exam call only the final test and the laboratory sessions evaluation test can be re-taken.
9. Assignments, course calendar and important dates
| Not related to the syllabus/contents | |
|---|---|
| Hours | hours |
10. Bibliography and Sources
